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内容大纲
组织工程是一个跨学科领域,旨在为各种应用创造功能性的组织和器官。本书全面概述组织工程实验策略,涵盖四个主要方面:细胞播种,支架制造,生长因子和基因传递,以及生物制造。各章实验展示了组织工程实验中的相关原理和技术,可为初学者和研究生提供基本信息。 -
作者介绍
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目录
Chapter 1 Seeding Cells
Experiment 1 Preparation of Induced Pluripotent Stem Cells
Experiment 2 Preparation of Embryonic Stem Cells
Experiment 3 Preparation of Mesenchymal Stem Cells
Experiment 4 Preparation of Placenta-Derived Mesenchymal Stem Cells
Experiment 5 Preparation of Adipose Mesenchymal Stem Cells
Experiment 6 Preparation of Endotheliocytes
Experiment 7 Preparation of Epithelial Cells
Experiment 8 Preparation of Fibroblasts
Experiment 9 Preparation of Osteocytes
Experiment 10 Preparation of Osteoblasts
Experiment 11 Preparation of Keratinocytes
Experiment 12 Preparation of Preadipocytes
Experiment 13 Preparation of Adipocytes
Experiment 14 Preparation of Tendon Cells
Chapter 2 New Biomaterials for Scaffolds in Regenerative Medicine
Experiment 15 Preparation of Poly (lactic-co-glycolic acid) Copolymer Scaffolds
Experiment 16 Preparation of Polyvinyl Alcohol Scaffolds
Experiment 17 Preparation of Polylysine Scaffolds
Experiment 18 Preparation of Chitosan Scaffolds
Experiment 19 Preparation of Gelatin Methacryloyl Scaffolds
Experiment 20 Preparation of Collagen Scaffolds
Experiment 21 Preparation of Alginate Scaffolds
Experiment 22 Preparation of Fibrinogen Scaffolds
Experiment 23 Preparation of Hyaluronic Acid Scaffolds
Experiment 24 Preparation of Alginate-Gelatin Scaffolds
Experiment 25 Preparation of Polyvinyl Alcohol-Gelatin Scaffolds
Chapter 3 Growth Factors and Gene Delivery for Regenerative Medicine
Experiment 26 Application of Nanoparticles for Growth Factor Delivery
Experiment 27 Application of Liposomes for Growth Factor Delivery
Experiment 28 Application of Gold Nanoparticles for Growth Factor Delivery
Experiment 29 Application of Responsive Hydrogels for Growth Factor Delivery
Experiment 30 Application of Gene Delivery in Regenerative Medicine
Chapter 4 Biofabrication in Regenerative Medicine
Experiment 31 Decellularization in Regenerative Medicine
Experiment 32 Recellularization in Regenerative Medicine
Experiment 33 Cell Sheet Engineering in Regenerative Medicine
Experiment 34 Extrusion-Based Bioprinting Application in Regenerative Medicine
Experiment 35 Droplet-Based Bioprinting or Inkjet Bioprinting in Regenerative Medicine
Experiment 36 Laser-Based Bioprinting Application in Regenerative Medicine
Experiment 37 Application of Lab-on-a-Chip in Regenerative Medicine
References
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